Biocompatibility Studies Of Quercetin Loaded Polymannose Nanoparticles For Potential Chemotherapeutic Application

Abstract

INTRODUCTION

Quercetin is a natural flavonoid known for its various pharmacological activities, but has poor solubility and bioavailability. Polymannose, a synthetic polymer obtained by the polymerization of mannose, can enhance solubility and is suitable for drug delivery.

AIM

To characterize quercetin loaded polymannose nanosheets (Qur-PM-NS) and investigate its biocompatibility for potential chemotherapeutic application.

MATERIALS AND METHODS:

Qur-PM-NS were prepared and characterized using UV-Vis spectrum, FT-IR and SEM. In vitro drug release study was also carried out to quantify the amount of quercetin resealed in particular time . Biocompatibility was determined by Annexin V PI assay.

RESULTS AND DISCUSSION

The UV-Vis spectrum of Qur-PM-NS gave a strong peak at 381 nm.  FT-IR of Qur-PM-NS gave peaks at  3293, 3260, 1649, 1602, 1533, 1451, 1312, 1272, 1160, 1039, 1006, 953, 795, 704, 635, 601 and 571 cm^-1.  3293 and 3260 cm^-1 indicating the presence of various functional groups. SEM analysis gave rod shaped morphology. The average drug release was found to be  3.6   % at 48h. Similarly, in the Annexin V PI assay 78.28% of cells were viable  and this is found to be significant.

CONCLUSION

Qur-PM-NS was found to be biocompatible and can be used for chemotherapeutic applications. This is to conclude that we have prepared biocompatible quercetin. loaded Polymannose nanofibres.