Effect Of Antioxidant Vitamins On Protein Kinase-C And Phosphotyrosine Phosphatase 1b Expression In The Liver Of Glyphosate - Induced Experimental Diabetic Rats

Abstract

INTRODUCTION: Antioxidant Activity is limitation of nutrient oxidation by restricting oxidative chain reaction. Studies showed that glyphosate and its formulated products are found to be carcinogenic. Supplementation with vitamin C and vitamin E influences the generation of free radicals and improves Antioxidant defenses.

AIM: Aim of the study is to analyze the effect of antioxidant vitamins on protein kinase C and Phosphotyrosine Phosphatase 1B in the liver of glyphosate induced diabetic rats.

METHODS: Adult male Albino rats were divided into 3 groups with 6 each.

Group 1: Control rats. Group 2: Glyphosate induced rats. Group 3: Glyphosate induced rats treated with vitamin C and vitamin E for 30 days.

Fasting blood glucose, serum insulin, protein kinase C mRNA and phosphotyrosine phosphatase 1B mRNA were studied and data were statistically analyzed using one-way-ANOVA.

RESULTS: Vitamin C and vitamin E showed a significant decrease (p<0.05) in fasting blood glucose, reduced serum insulin concentration compared to control which was altered by glyphosate exposure. mRNA levels of protein kinase C compared to and phosphotyrosine phosphatase 1B were significantly raised in the glyphosate-exposed diabetic group compared to the healthy group. Vitamin supplementation significantly reduced the same effectively (p<0.05).

CONCLUSION: Vitamin C and vitamin E shows significant Anti-diabetic Activity by decreasing levels of fasting blood glucose and serum insulin and by altering gene expression of protein kinase C and phosphotyrosine phosphatase 1B