Study on Effective of Bacillus subtilis for Domestic Wastewater Treatment in the Royal Thai Army Chemical Department Area

Abstract

Recently, wastewater has been discharged from various sources into natural water sources, causing them to be contaminated with waste. Therefore, this study concentrates on guidelines for utilizing Bacillus Subtilis for wastewater treatment in the pond in front of the Science Division, Department of Army Science, which collects wastewater from office buildings and accommodations within the Royal Thai Army Science Department and discharges it to the public canal. Therefore, water must be treated before discharge. Samples were collected from a pond in front of the Science Division and a pond in front of the Pyro building for bacterial isolation (two soil samples and two water samples). The isolated and purified bacteria were used for preliminary morphological studies. All the isolated bacteria were gram-negative bacteria with 21 endospores. Protein, starch, and lipid degradation enzymes were tested using selected foods, namely skimmed milk agar, starch agar, and Tween 80 agar, and the point inoculation technique was applied. The results revealed that the isolates KCT03, KCT04, and KCT05 could produce these three groups of enzymes. The antagonism of the isolated bacteria was tested, and it was found that the three species were not antagonistic. All three samples were classified as bacteria, and KCT03 and KCT05 were found to be Bacillus subtilis. The efficiency of microbial wastewater treatment was tested at the laboratory level, and the experiment was planned to be a completely randomized design (CRD) consisting of three experimental sets and three repetitions to measure the water quality, pH, COD, BOD, TSS, TDS, oil, and fat. It was found that KCT03 was the best wastewater treatment.