Copper Sulfate-Induced Alterations In Growth Parameters And Tissue Copper Levels In Rohtee Ogilbii

Abstract

Ten Rohtee Ogilbii fish were placed in each of twelve glass aquariums and subjected to different amounts of copper sulfate (25 µg/L, 50 µg/L, and 75 µg/L), with control groups also being immersed in dechlorinated tap water. The fish started off with a body weight of 50 ± 2 g. Over the course of three sample days, the amounts of copper were recorded both before and after the water was renewed. Every day, the temperature, pH, conductivity, and unionized ammonia levels in the water were just right for the fish to thrive. Liver weights were assessed before freezing, and tissue samples (liver, gills, and muscle) were obtained and preserved for examination. The computed growth parameters demonstrated that the effects of copper sulfate on growth and tissue accumulation were significantly dose-dependent. There were significant differences (p < 0.05) across the treatment groups, as shown by statistical analysis using ANOVA and Duncan's multiple range tests. The results show that copper sulfate has physiological effects on Rohtee Ogilbii, which means that copper levels in water need to be carefully managed to prevent harmful effects on fish health and development.