Evaluating A Hands-On Training Program For Improving Laboratory Technicians’ Skills In Molecular Diagnostic Techniques

Abstract

Molecular diagnostic techniques like polymerase chain reaction (PCR) are becoming essential for clinical laboratories. However, many technicians have insufficient hands-on training and experience performing these techniques. This study evaluated a two-day intensive PCR training program for laboratory technicians in Riyadh, Saudi Arabia. A mixed methods approach collected pre/post-training surveys, knowledge tests, self-efficacy ratings, and qualitative interviews. Participants included 24 laboratory technicians from six hospitals undergoing the training. Knowledge improved significantly from mean of 53.8% to 88.9% (p<0.001). Self-efficacy increased from mean of 3.2 to 4.6 out of 5 (p<0.001). Participants described enhanced technical and troubleshooting abilities, though desired further hands-on time. Survey analysis and interviews suggest key program strengths were expert trainers, practical demonstrations, and small group work. Weaknesses included short duration and need for individual technique practice. Overall, this targeted hands-on PCR training program significantly improved technicians’ knowledge, skills, and confidence. Study outcomes can guide enhancements to laboratory molecular training programs in Saudi Arabia and globally, helping expand staff capabilities as molecular diagnostics diffuse into routine care.