In Vivo Study Of Hypersensitivity And Irritation Skin For Nanocomposite Biphasic Calcium Phosphate Synthesized Directly By Hydrothermal Process

Abstract

Mimicking the natural structure of bone, like nanomaterial, is an attractive process for bone regeneration. A calcium phosphate biphasic (BCP), assembling the hydroxyapatite (HA) and β-tricalcium phosphate (β- TCP), these two compounds are used as bone substitutes to its physical and chemical properties very close to that of the mineral phase of the bone. They are exploited for their outstanding biocompatibility and bioactivity. This research aims to synthesize a calcium phosphate biphasic directly by hydrothermal method and estimate the inflammatory potential in vivo using a histological study in mice. The calcium acetate (CH₃COO) ₂Ca and the phosphoric acid H₃PO₄ were used as a precursor of Ca and P in the presence of triethylamine N (CH₂CH₃)₃. The BCP ceramics were characterized by techniques for structural and morphological characterizations SEM, ICP, IR, and XRD (ATG/ATD). To affirm their phase content. It was confirmed that the main inorganics were hydroxyapatite and tricalcium phosphate with grain sizes below 50 nm. BCP in vivo assays exhibits non-cytotoxic cell proliferation and cell adhesion.