Comparing Chlorophyll Extraction Methods in Tiliacora triandra (Colebr)

Abstract

Tiliacora triandra (Colebr) is a plant known for its traditional medicinal and culinary uses. The leaves of this plant are commonly consumed as a vegetable or used in the preparation of herbal beverages. Chlorophyll, a green pigment found in plants, is known to contain antioxidants and vitamins. This study aimed to analyze the total chlorophyll content in Tiliacora triandra using different extraction methods, namely acetone, methanol, ethanol, and water. The objective was to determine the most effective extraction method for obtaining accurate results.Fresh leaves of Tiliacora triandra were collected and subjected to four different extraction methods: acetone, methanol, ethanol, and water. The leaves were macerated and the extracts were obtained by filtration. The total chlorophyll content in each extract was measured using spectrophotometric analysis at specific wavelengths. The extraction process was performed for a duration of 30 minutes.The results indicated that the ethanol extraction method yielded the highest total chlorophyll content compared to the other three methods (acetone, methanol, and water). This suggests that ethanol is the most efficient solvent for extracting chlorophyll from Tiliacora triandra leaves. The superiority of the ethanol extraction method can be attributed to its ability to effectively solubilize chlorophyll pigments. Ethanol has a higher affinity for chlorophyll compared to acetone, methanol, and water, resulting in a more efficient extraction process. The 30-minute extraction duration was found to be optimal for obtaining the maximum chlorophyll content.Chlorophyll extraction plays a crucial role in investigating the properties and potential therapeutic effects of this compound. By obtaining pure chlorophyll extracts, researchers can analyze its chemical composition, explore its biological functions, and evaluate its potential applications in various fields.In conclusion, the ethanol extraction method for a duration of 30 minutes proved to be the most effective for obtaining the highest total chlorophyll content in Tiliacora triandra (Colebr). This information is valuable for future studies and applications involving chlorophyll in this plant species. Proper selection of the extraction method is essential to preserve the purity and quality of the extracted chlorophyll, depending on the intended end-use. Further research can focus on harnessing the potential therapeutic benefits of chlorophyll from Tiliacora triandra in various environmental and health-related applications.